Culture of Epithelial Cells 2nd Edition

"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." —Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.Content: Chapter 1 Introduction (pages 1–30): R. Ian FreshneyChapter 2 Cell Interaction and Epithelial Differentiation (pages 31–63): Nicole Maas?Szabowski, Hans?Jurgen Stark and Norbert E. FusenigChapter 3 The Epidermis (pages 65–94): E. Kenneth Parkinson and W. Andrew YeudallChapter 4 Culture of Human Mammary Epithelial Cells (pages 95–135): Martha R. Stampfer, Paul Yaswen and Joyce Taylor?PapadimitriouChapter 5 Culture of Human Cervical Epithelial Cells (pages 137–169): Margaret A. StanleyChapter 6 Human Prostatic Epithelial Cells (pages 171–194): Donna M. PeehlChapter 7 Human Oral Epithelium (pages 195–255): Roland C. GrafstromChapter 8 Normal Human Bronchial Epithelial Cell Culture (pages 257–276): John Wise and John F. LechnerChapter 9 Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells (pages 277–301): Leland G. Dobbs and Robert F. GonzalezChapter 10 Isolation and Culture of Intestinal Epithelial Cells (pages 303–335): Catherine Booth and Julie A. O'SheaChapter 11 Isolation and Culture of Animal and Human Hepatocytes (pages 337–379): Christiane Guguen?GuillouzoChapter 12 Culture of Human Urothelium (pages 381–399): Jennifer Southgate, John R.W. Masters and Ludwik K. TrejdosiewiczChapter 13 Other Epithelial Cells (pages 401–436): R. Ian Freshney